Selector-technique

The selector technique is a method to amplify and multiplex genomic DNA. Process Genomic DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence by ligation. The procedure results in circular DNA molecules with an included general primer pair motif that can be used for amplification by PCR or RCA. Selector construct A selector consists of two oligonucleotides, one Vector oligonucleotide and one Selector probe. Together they form one Selector with target specific ends on each side of a general primer motif.